Developing high affinity antibodies as diagnostics

Monoclonal Antibodies as Diagnostics; an Appraisal

Ever since the development of Hybridoma Technology in 1975 by Kohler and Milstein, our vision for antibodies as tools for research for prevention, detection and treatment of diseases, vaccine production, antigenic characterization of pathogens and in the study of genetic regulation of immune responses and disease susceptibility has been revolutionized. The monoclonal antibodies being directed a...

Diagnostics for Developing Countries

Improving the availability of high quality diagnostic tests for infectious diseases is a global priority. Lack of access by people living in low income countries may deprive them of life saving treatment and reduces opportunities to prevent onward transmission and spread of the disease. Diagnostic laboratories are often poorly resourced in developing countries, and sparsely distributed. Improve...

MALT lymphoma-derived rheumatoid factors are nonpolyreactive high-affinity antibodies.

High-Affinity Rabbit Monoclonal Antibodies Specific for Amyloid Peptides

Antibodies that specifically bind to either amyloidpeptide (A ) isoform A 40 or A 42 contribute to the study of Alzheimer’s disease (AD) pathology and to the development of cerebrospinal fluid-based tests for the probable diagnosis of AD. Polyclonal rabbit anti-A antibodies possess high affinity and specificity, but their generation requires a long immunization period, and the resulting antibod...

Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies.

High-affinity monoclonal IgG and IgM antibodies to hepatitis B surface antigen (HBsAg) have been prepared and their functional capabilities explored by means of solid-phase radioimmunoassays. 125I-labeled HBsAg binding studies indicated that monoclonal IgM antibodies against HBsAg (anti-HBs) coupled to a solid-phase support quantitatively bound more HbsAg at a faster rate than conventionally pr...